PDBe 3bl2

X-ray diffraction
2.3Å resolution

Crystal Structure of M11, the BCL-2 Homolog of Murine Gamma-herpesvirus 68, Complexed with Mouse Beclin1 (residues 106-124)

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Apoptosis regulator Bcl-2 homolog Chains: A, B
Molecule details ›
Chains: A, B
Length: 131 amino acids
Theoretical weight: 15.15 KDa
Source organism: Murid gammaherpesvirus 4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P89884 (Residues: 5-135; Coverage: 77%)
Gene name: vBCL2
Sequence domains: Apoptosis regulator M11, B cell 2 leukaemia/lymphoma like
Structure domains: Blc2-like
Beclin-1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 19 amino acids
Theoretical weight: 2.24 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O88597 (Residues: 106-124; Coverage: 4%)
Gene name: Becn1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: P21
Unit cell:
a: 42.863Å b: 53.601Å c: 73.586Å
α: 90° β: 107.54° γ: 90°
R-values:
R R work R free
0.204 0.204 0.247
Expression system: Escherichia coli BL21(DE3)