3bjx

X-ray diffraction
2.3Å resolution

Structure of a Group I haloacid dehalogenase from Pseudomonas putida strain PP3

Released:

Function and Biology Details

Reaction catalysed:
(S)-2-haloacid + H(2)O = (R)-2-hydroxyacid + halide
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Halocarboxylic acid dehalogenase DehI Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 311 amino acids
Theoretical weight: 34.87 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8GJ84 (Residues: 1-296; Coverage: 100%)
Gene name: dehI
Sequence domains: Halocarboxylic acid dehydrogenase DehI

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU, SSRL BEAMLINE BL9-1
Spacegroup: P21
Unit cell:
a: 68.323Å b: 111.864Å c: 75.181Å
α: 90° β: 93.71° γ: 90°
R-values:
R R work R free
0.185 0.181 0.25
Expression system: Escherichia coli