3bdk

X-ray diffraction
2.5Å resolution

Crystal Structure of Streptococcus suis mannonate dehydratase complexed with substrate analogue

Released:
Source organism: Streptococcus suis
Entry authors: Gao F, Zhang QM, Peng H, Liu YW, Qi JX, Gao GF

Function and Biology Details

Reaction catalysed:
D-mannonate = 2-dehydro-3-deoxy-D-gluconate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mannonate dehydratase Chains: A, B
Molecule details ›
Chains: A, B
Length: 386 amino acids
Theoretical weight: 43.02 KDa
Source organism: Streptococcus suis
Expression system: Escherichia coli
UniProt:
  • Canonical: A4VVI4 (Residues: 1-366; Coverage: 100%)
Gene names: SSU05_1157, uxuA
Sequence domains: D-mannonate dehydratase (UxuA)
Structure domains: Divalent-metal-dependent TIM barrel enzymes

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P43212
Unit cell:
a: 105.468Å b: 105.468Å c: 160.155Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.253 0.25 0.303
Expression system: Escherichia coli