3bcm

X-ray diffraction
2.25Å resolution

Crystal Structure of The Unswapped Form of P19A/L28Q/N67D BS-RNase

Released:

Function and Biology Details

Reaction catalysed:
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132818 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Seminal ribonuclease Chains: A, B
Molecule details ›
Chains: A, B
Length: 124 amino acids
Theoretical weight: 13.62 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00669 (Residues: 27-150; Coverage: 100%)
Gene name: SRN
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P21
Unit cell:
a: 48.513Å b: 59.508Å c: 49.738Å
α: 90° β: 118.02° γ: 90°
R-values:
R R work R free
0.224 0.183 0.252
Expression system: Escherichia coli