X-ray diffraction
2.35Å resolution

Structure of the eEF2-ExoA(E546H)-NAD+ complex


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Exotoxin A Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 207 amino acids
Theoretical weight: 22.45 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
  • Canonical: P11439 (Residues: 425-630; Coverage: 34%)
Gene names: PA1148, eta
Sequence domains: Exotoxin A catalytic
Structure domains: Diphtheria Toxin, domain 1

Ligands and Environments

Cofactor: Ligand NAD 3 x NAD
No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: C2
Unit cell:
a: 327.57Å b: 68.46Å c: 189.99Å
α: 90° β: 103.04° γ: 90°
R R work R free
0.215 0.215 0.257
Expression system: Escherichia coli