3b78

X-ray diffraction
2.5Å resolution

Structure of the eEF2-ExoA(R551H)-NAD+ complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Exotoxin A Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 207 amino acids
Theoretical weight: 22.42 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P11439 (Residues: 425-630; Coverage: 34%)
Gene names: PA1148, eta
Sequence domains: Exotoxin A catalytic
Structure domains: Diphtheria Toxin, domain 1

Ligands and Environments


Cofactor: Ligand NAD 3 x NAD
No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 327.136Å b: 68.128Å c: 190.577Å
α: 90° β: 102.994° γ: 90°
R-values:
R R work R free
0.206 0.206 0.242
Expression system: Escherichia coli