› 3b61

X-ray diffraction
4.5Å resolution

EmrE multidrug transporter, apo crystal form

Released:
DOI: 10.2210/pdb3b61/pdb
PDB entry 3b61 coloured by chain, front view.
PDB entry 3b61 coloured by chain, side view.
PDB entry 3b61 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
X-ray structure of EmrE supports dual topology model.
Chen YJ, Pornillos O, Lieu S, Ma C, Chen AP, Chang G
Proc Natl Acad Sci U S A 104 18999-9004 (2007)
PMID: 18024586

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150097 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multidrug transporter EmrE Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 110 amino acids
Theoretical weight: 11.96 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P23895 (Residues: 1-110; Coverage: 100%)
Gene names: JW0531, b0543, eb, emrE, mvrC
Sequence domains: Small Multidrug Resistance protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: F222
Unit cell:
a: 181Å b: 239.2Å c: 284.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.318 0.318 0.362
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

51 review citations

Efflux-mediated drug resistance in bacteria: an update.
Li et al. (2009)
50 more

9 mentions without citation

Modeling kinetics of subcellular disposition of chemicals.
Balaz S. (2009)
8 more