3b39

X-ray diffraction
2.35Å resolution

Structure of the DnaG primase catalytic domain bound to ssDNA

Released:
Source organism: Escherichia coli K-12

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA primase Chains: A, B
Molecule details ›
Chains: A, B
Length: 322 amino acids
Theoretical weight: 36.29 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABS5 (Residues: 111-429; Coverage: 55%)
Gene names: JW3038, b3066, dnaG, dnaP, parB
Sequence domains:
Structure domains:
DNA (5'-D(*DCP*DAP*DAP*DAP*DGP*DCP*DCP*DAP*DAP*DAP*DAP*DGP*DGP*DAP*DC)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 15 nucleotides
Theoretical weight: 4.61 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P62
Unit cell:
a: 136.829Å b: 136.829Å c: 71.071Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.212 0.254
Expression systems:
  • Escherichia coli
  • Not provided