3b0d

X-ray diffraction
2.2Å resolution

Crystal structure of the chicken CENP-T histone fold/CENP-W complex, crystal form II

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Centromere protein T Chains: B, T
Molecule details ›
Chains: B, T
Length: 111 amino acids
Theoretical weight: 12.75 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: F1NPG5 (Residues: 531-639; Coverage: 17%)
Gene name: CENPT
Structure domains: Histone, subunit A
Centromere protein W Chains: C, W
Molecule details ›
Chains: C, W
Length: 76 amino acids
Theoretical weight: 8.71 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DJH6 (Residues: 2-76; Coverage: 99%)
Gene name: CENPW
Structure domains: Histone, subunit A

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P3121
Unit cell:
a: 54.985Å b: 54.985Å c: 235.912Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.208 0.203 0.261
Expression system: Escherichia coli