3akc

X-ray diffraction
1.65Å resolution

Crystal structure of CMP kinase in complex with CDP and ADP from Thermus thermophilus HB8

Released:
Source organism: Thermus thermophilus HB8
Entry authors: Mega R, Nakagawa N, Kuramitsu S, Masui R

Function and Biology Details

Reaction catalysed:
ATP + (d)CMP = ADP + (d)CDP
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytidylate kinase Chain: A
Molecule details ›
Chain: A
Length: 208 amino acids
Theoretical weight: 22.58 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SL35 (Residues: 1-208; Coverage: 100%)
Gene names: TTHA0458, cmk
Sequence domains: Cytidylate kinase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL45XU
Spacegroup: P65
Unit cell:
a: 62.206Å b: 62.206Å c: 110.951Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.199 0.22
Expression system: Escherichia coli