› 3ai5

X-ray diffraction
1.4Å resolution

Crystal structure of yeast enhanced green fluorescent protein-ubiquitin fusion protein

Released:
DOI: 10.2210/pdb3ai5/pdb
PDB entry 3ai5 coloured by chain, front view.
PDB entry 3ai5 coloured by chain, side view.
PDB entry 3ai5 coloured by chain, top view.
Source organisms:
Primary publication:
Crystallization of small proteins assisted by green fluorescent protein.
Suzuki N, Hiraki M, Yamada Y, Matsugaki N, Igarashi N, Kato R, Dikic I, Drew D, Iwata S, Wakatsuki S, Kawasaki M
Acta Crystallogr D Biol Crystallogr 66 1059-66 (2010)
PMID: 20944239

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154511 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein; Ubiquitin Chain: A
Molecule details ›
Chain: A
Length: 307 amino acids
Theoretical weight: 34.75 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 1-230; Coverage: 97%)
  • Canonical: P62984 (Residues: 1-74; Coverage: 58%)
Gene names: GFP, Uba52, Ubcep2
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand EDO 5 x EDO
1 modified residue:
Ligand CR2 1 x CR2

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: C2
Unit cell:
a: 92.515Å b: 65.649Å c: 70.225Å
α: 90° β: 130.34° γ: 90°
R-values:
R R work R free
0.18 0.179 0.209
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Unraveling aquaporin interaction partners.
Sjöhamn et al. (2014)
2 more

1 mention without citation

Fusion of two unrelated protein domains in a chimera protein and its 3D prediction: Justification of the x-ray reference structures as a prediction benchmark.
Vymětal et al. (2022)