3aam

X-ray diffraction
1.58Å resolution

Crystal structure of endonuclease IV from Thermus thermophilus HB8

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AP_endonuc_2 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 270 amino acids
Theoretical weight: 29.13 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SK18 (Residues: 1-270; Coverage: 100%)
Gene name: TTHA0834
Sequence domains: Xylose isomerase-like TIM barrel
Structure domains: Divalent-metal-dependent TIM barrel enzymes

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P21
Unit cell:
a: 36.646Å b: 85.273Å c: 36.58Å
α: 90° β: 105.37° γ: 90°
R-values:
R R work R free
0.199 0.199 0.223
Expression system: Escherichia coli BL21(DE3)