› 3a6v

X-ray diffraction
2Å resolution

Crystal structure of the MutT protein in MN(II) bound holo form

Released:
DOI: 10.2210/pdb3a6v/pdb
PDB entry 3a6v coloured by chain, front view.
PDB entry 3a6v coloured by chain, side view.
PDB entry 3a6v coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural and dynamic features of the MutT protein in the recognition of nucleotides with the mutagenic 8-oxoguanine base.
Nakamura T, Meshitsuka S, Kitagawa S, Abe N, Yamada J, Ishino T, Nakano H, Tsuzuki T, Doi T, Kobayashi Y, Fujii S, Sekiguchi M, Yamagata Y
J Biol Chem 285 444-52 (2010)
PMID: 19864691

Function and Biology Details

Reaction catalysed: 
8-oxo-dGTP + H(2)O = 8-oxo-dGMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-140099 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
8-oxo-dGTP diphosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 129 amino acids
Theoretical weight: 14.95 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08337 (Residues: 1-129; Coverage: 100%)
Gene names: JW0097, b0099, mutT
Sequence domains: NUDIX domain
Structure domains: Nucleoside Triphosphate Pyrophosphohydrolase

Ligands and Environments

3 bound ligands:
Ligand MN 4 x MN
Ligand NA 2 x NA
Ligand TLA 3 x TLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-18B
Spacegroup: P21
Unit cell:
a: 35.75Å b: 55.95Å c: 74.12Å
α: 90° β: 96.4° γ: 90°
R-values:
R R work R free
0.192 0.192 0.227
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

The BER necessities: the repair of DNA damage in human-adapted bacterial pathogens.
van der Veen et al. (2015)
2 more

2 mentions without citation

Structural and dynamic features of the MutT protein in the recognition of nucleotides with the mutagenic 8-oxoguanine base.
Nakamura et al. (2010)
1 more