3a1q

X-ray diffraction
2.2Å resolution

Crystal structure of the mouse RAP80 UIMs in complex with Lys63-linked di-ubiquitin

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ubiquitin Chains: A, D
Molecule details ›
Chains: A, D
Length: 77 amino acids
Theoretical weight: 8.69 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG50 (Residues: 609-684; Coverage: 10%)
Gene name: Ubc
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Ubiquitin Chains: B, E
Molecule details ›
Chains: B, E
Length: 76 amino acids
Theoretical weight: 8.6 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG50 (Residues: 609-684; Coverage: 10%)
Gene name: Ubc
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
BRCA1-A complex subunit RAP80 Chains: C, F
Molecule details ›
Chains: C, F
Length: 45 amino acids
Theoretical weight: 5.08 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5U5Q9 (Residues: 80-120; Coverage: 6%)
Gene names: Rap80, Rip110, Rxrip110, Uimc1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 65.235Å b: 74.81Å c: 83.031Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.216 0.268
Expression system: Escherichia coli