301d

X-ray diffraction
3Å resolution

CAPTURING THE STRUCTURE OF A CATALYTIC RNA INTERMEDIATE: RNA HAMMERHEAD RIBOZYME, MG(II)-SOAKED

Released:
Source organism: Synthetic construct
Entry authors: Scott WG, Murray JB, Arnold JRP, Stoddard BL, Klug A

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
RNA HAMMERHEAD RIBOZYME Chain: A
Molecule details ›
Chain: A
Length: 16 nucleotides
Theoretical weight: 5.17 KDa
Source organism: Synthetic construct
Expression system: Not provided
RNA HAMMERHEAD RIBOZYME Chain: B
Molecule details ›
Chain: B
Length: 25 nucleotides
Theoretical weight: 8.02 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX7.2
Spacegroup: P3121
Unit cell:
a: 65.9Å b: 65.9Å c: 136.77Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.258 0.258 0.297
Expression system: Not provided