› 301d

X-ray diffraction
3Å resolution

CAPTURING THE STRUCTURE OF A CATALYTIC RNA INTERMEDIATE: RNA HAMMERHEAD RIBOZYME, MG(II)-SOAKED

Released:
DOI: 10.2210/pdb301d/pdb
PDB 301d coloured by chain and viewed from the front.
PDB 301d coloured by chain and viewed from the side.
PDB 301d coloured by chain and viewed from the top.
Source organism: Synthetic construct
Entry authors: Scott WG, Murray JB, Arnold JRP, Stoddard BL, Klug A

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
RNA HAMMERHEAD RIBOZYME Chain: A
Molecule details ›
Chain: A
Length: 16 nucleotides
Theoretical weight: 5.17 KDa
Source organism: Synthetic construct
Expression system: Not provided
RNA HAMMERHEAD RIBOZYME Chain: B
Molecule details ›
Chain: B
Length: 25 nucleotides
Theoretical weight: 8.02 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 5 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX7.2
Spacegroup: P3121
Unit cell:
a: 65.9Å b: 65.9Å c: 136.77Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.258 0.258 0.297
Expression system: Not provided

Quick links

Citations

70 review citations

Molecular Features and Metal Ions That Influence 10-23 DNAzyme Activity.
Rosenbach et al. (2020)
69 more

4 mentions without citation

Comparison of mode analyses at different resolutions applied to nucleic acid systems.
Van Wynsberghe et al. (2005)
3 more