› 300d

X-ray diffraction
3Å resolution

CAPTURING THE STRUCTURE OF A CATALYTIC RNA INTERMEDIATE: RNA HAMMERHEAD RIBOZYME, MN(II)-SOAKED

Released:
DOI: 10.2210/pdb300d/pdb
PDB entry 300d coloured by chain, front view.
PDB entry 300d coloured by chain, side view.
PDB entry 300d coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Capturing the structure of a catalytic RNA intermediate: the hammerhead ribozyme.
Scott WG, Murray JB, Arnold JR, Stoddard BL, Klug A
Science 274 2065-9 (1996)
PMID: 8953035

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196610 (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
RNA HAMMERHEAD RIBOZYME Chain: A
Molecule details ›
Chain: A
Length: 16 nucleotides
Theoretical weight: 5.17 KDa
Source organism: Synthetic construct
Expression system: Not provided
RNA HAMMERHEAD RIBOZYME Chain: B
Molecule details ›
Chain: B
Length: 25 nucleotides
Theoretical weight: 8.02 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MN 6 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P3121
Unit cell:
a: 64.55Å b: 64.55Å c: 135.87Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.217 0.262
Expression system: Not provided

Quick links

Citations

70 review citations

The chemical repertoire of natural ribozymes.
Doudna et al. (2002)
69 more

15 mentions without citation

General Strategies for RNA X-ray Crystallography.
Jackson et al. (2023)
14 more