X-ray diffraction
2.3Å resolution

Crystal structure of 3-ketosteroid-9-alpha-hydroxylase (KshA) from M. tuberculosis


Function and Biology Details

Reaction catalysed:
Androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H(+) + O(2) = 9-alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
3-ketosteroid-9-alpha-monooxygenase, oxygenase component Chain: A
Molecule details ›
Chain: A
Length: 386 amino acids
Theoretical weight: 44.33 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
  • Canonical: P71875 (Residues: 1-386; Coverage: 100%)
Gene names: Rv3526, kshA
Sequence domains: Rieske [2Fe-2S] domain
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P321
Unit cell:
a: 116.2Å b: 116.2Å c: 81Å
α: 90° β: 90° γ: 120°
R R work R free
0.199 0.197 0.235
Expression system: Escherichia coli