› 2zxt

X-ray diffraction
3Å resolution

Crystal structure of Tim40/MIA40, a disulfide relay system in mitochondria, solved as MBP fusion protein

Released:
DOI: 10.2210/pdb2zxt/pdb
PDB entry 2zxt coloured by chain, front view.
PDB entry 2zxt coloured by chain, side view.
PDB entry 2zxt coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of yeast Tim40/Mia40 as an oxidative translocator in the mitochondrial intermembrane space.
Kawano S, Yamano K, Naoé M, Momose T, Terao K, Nishikawa S, Watanabe N, Endo T
Proc Natl Acad Sci U S A 106 14403-7 (2009)
PMID: 19667201

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142533 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Maltose/maltodextrin-binding periplasmic protein; Mitochondrial intermembrane space import and assembly protein 40 Chain: A
Molecule details ›
Chain: A
Length: 465 amino acids
Theoretical weight: 51.36 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 27-392; Coverage: 99%)
  • Canonical: P36046 (Residues: 267-365; Coverage: 25%)
Gene names: JW3994, MIA40, TIM40, YKL195W, b4034, malE
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P212121
Unit cell:
a: 41.263Å b: 101.403Å c: 109.355Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.205 0.278
Expression system: Escherichia coli

Quick links

Citations

28 review citations

Mitochondrial protein import: from proteomics to functional mechanisms.
Schmidt et al. (2010)
27 more

5 mentions without citation

Chemistry and Enzymology of Disulfide Cross-Linking in Proteins.
Fass et al. (2018)
4 more