2zw9

X-ray diffraction
2.5Å resolution

Crystal structure of tRNA wybutosine synthesizing enzyme TYW4

Released:

Function and Biology Details

Reactions catalysed:
S-adenosyl-L-methionine + 7-((3S)-3-amino-3-carboxypropyl)wyosine(37) in tRNA(Phe) = S-adenosyl-L-homocysteine + 7-((3S)-3-amino-3-(methoxycarbonyl)propyl)wyosine(37) in tRNA(Phe)
S-adenosyl-L-methionine + 7-((3S)-3-amino-3-(methoxycarbonyl)propyl)wyosine(37) in tRNA(Phe) + CO(2) = S-adenosyl-L-homocysteine + wybutosine(37) in tRNA(Phe)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA wybutosine-synthesizing protein 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 695 amino acids
Theoretical weight: 79.05 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q08282 (Residues: 1-695; Coverage: 100%)
Gene names: PPM2, TYW4, YOL141W
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand SAM 2 x SAM
No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 70.442Å b: 88.628Å c: 256.115Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.193 0.245
Expression system: Escherichia coli