› 2zuq

X-ray diffraction
3.3Å resolution

Crystal structure of DsbB-Fab complex

Released:
DOI: 10.2210/pdb2zuq/pdb
PDB 2zuq coloured by chain and viewed from the front.
PDB 2zuq coloured by chain and viewed from the side.
PDB 2zuq coloured by chain and viewed from the top.
Source organisms:
Entry authors: Inaba K, Suzuki M, Murakami S

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Disulfide bond formation protein B Chains: A, D
Molecule details ›
Chains: A, D
Length: 176 amino acids
Theoretical weight: 20.11 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6M2 (Residues: 1-176; Coverage: 100%)
Gene names: JW5182, b1185, dsbB, roxB, ycgA
Sequence domains: Disulfide bond formation protein DsbB
Structure domains: DsbB-like
Fab fragment light chain Chains: B, E
Molecule details ›
Chains: B, E
Length: 239 amino acids
Theoretical weight: 26.36 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Fab fragment heavy chain Chains: C, F
Molecule details ›
Chains: C, F
Length: 221 amino acids
Theoretical weight: 23.67 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand UQ1 2 x UQ1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: C2
Unit cell:
a: 269.307Å b: 51.48Å c: 125.845Å
α: 90° β: 106.89° γ: 90°
R-values:
R R work R free
0.279 0.275 0.351
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Structural bioinformatic analysis of DsbA proteins and their pathogenicity associated substrates.
Santos-Martin et al. (2021)
21 more

10 mentions without citation

Disulfide bond formation in the bacterial periplasm: major achievements and challenges ahead.
Denoncin et al. (2013)
9 more