2zui

X-ray diffraction
1.5Å resolution

Crystal Structure of Camphor-soaked Ferric Cytochrome P450cam Mutant (D297N)

Released:
Source organism: Pseudomonas putida
Entry authors: Sakurai K, Harada K, Shimada H, Shimokata K, Hayashi T, Tsukihara T

Function and Biology Details

Reaction catalysed:
(+)-camphor + reduced putidaredoxin + O(2) = (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H(2)O
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Camphor 5-monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 415 amino acids
Theoretical weight: 46.72 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: P00183 (Residues: 1-415; Coverage: 100%)
Gene names: camC, cyp101
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P43212
Unit cell:
a: 63.604Å b: 63.604Å c: 250.105Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.158 0.178
Expression system: Escherichia coli