2zu7

X-ray diffraction
2.5Å resolution

Crystal structure of mannosyl-3-phosphoglycerate synthase from Pyrococcus horikoshii

Released:
Source organism: Pyrococcus horikoshii
Entry authors: Kawamura T, Watanabe N, Tanaka I

Function and Biology Details

Reaction catalysed:
GDP-mannose + 3-phospho-D-glycerate = GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mannosyl-3-phosphoglycerate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 394 amino acids
Theoretical weight: 45.98 KDa
Source organism: Pyrococcus horikoshii
Expression system: Escherichia coli
UniProt:
  • Canonical: O58689 (Residues: 1-394; Coverage: 100%)
Gene names: PH0927, mngA
Sequence domains: Mannosyl-3-phosphoglycerate synthase (osmo_MPGsynth)
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 55.075Å b: 86.923Å c: 83.328Å
α: 90° β: 101.78° γ: 90°
R-values:
R R work R free
0.21 0.21 0.256
Expression system: Escherichia coli