2ztk

X-ray diffraction
1.96Å resolution

Crystal structure of homocitrate synthase from Thermus thermophilus complexed with homocitrate

Released:

Function and Biology Details

Reaction catalysed:
Acetyl-CoA + H(2)O + 2-oxoglutarate = (R)-2-hydroxybutane-1,2,4-tricarboxylate + CoA
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Homocitrate synthase Chain: A
Molecule details ›
Chain: A
Length: 382 amino acids
Theoretical weight: 43.07 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: O87198 (Residues: 1-376; Coverage: 100%)
Gene names: TT_C1550, lys20
Sequence domains: HMGL-like
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P6322
Unit cell:
a: 135.508Å b: 135.508Å c: 127.062Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.193 0.222
Expression system: Escherichia coli