2zsh

X-ray diffraction
1.8Å resolution

Structural basis of gibberellin(GA3)-induced DELLA recognition by the gibberellin receptor

Released:
Source organism: Arabidopsis thaliana

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Gibberellin receptor GID1A Chain: A
Molecule details ›
Chain: A
Length: 351 amino acids
Theoretical weight: 39.28 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9MAA7 (Residues: 1-344; Coverage: 100%)
Gene names: At3g05120, CXE10, GID1A, GID1L1, T12H1.8
Sequence domains: alpha/beta hydrolase fold
Structure domains: Rossmann fold
DELLA protein GAI Chain: B
Molecule details ›
Chain: B
Length: 110 amino acids
Theoretical weight: 12.28 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9LQT8 (Residues: 11-113; Coverage: 19%)
Gene names: At1g14920, F10B6.34, GAI, RGA2
Sequence domains: Transcriptional regulator DELLA protein N terminal
Structure domains: Arc Repressor Mutant, subunit A

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P43212
Unit cell:
a: 82.018Å b: 82.018Å c: 130.08Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.205 0.229
Expression system: Escherichia coli