› 2zsh

X-ray diffraction
1.8Å resolution

Structural basis of gibberellin(GA3)-induced DELLA recognition by the gibberellin receptor

Released:
DOI: 10.2210/pdb2zsh/pdb
PDB 2zsh coloured by chain and viewed from the front.
PDB 2zsh coloured by chain and viewed from the side.
PDB 2zsh coloured by chain and viewed from the top.
Source organism: Arabidopsis thaliana
Primary publication:
Gibberellin-induced DELLA recognition by the gibberellin receptor GID1.
Murase K, Hirano Y, Sun TP, Hakoshima T
Nature 456 459-63 (2008)
PMID: 19037309

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Gibberellin receptor GID1A Chain: A
Molecule details ›
Chain: A
Length: 351 amino acids
Theoretical weight: 39.28 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9MAA7 (Residues: 1-344; Coverage: 100%)
Gene names: At3g05120, CXE10, GID1A, GID1L1, T12H1.8
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase
DELLA protein GAI Chain: B
Molecule details ›
Chain: B
Length: 110 amino acids
Theoretical weight: 12.28 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9LQT8 (Residues: 11-113; Coverage: 19%)
Gene names: At1g14920, F10B6.34, GAI, RGA2
Sequence domains: Transcriptional regulator DELLA protein N terminal
Structure domains: Transcriptional regulator DELLA, N-terminal domain

Ligands and Environments

1 bound ligand:
Ligand GA3 1 x GA3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P43212
Unit cell:
a: 82.018Å b: 82.018Å c: 130.08Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.205 0.229
Expression system: Escherichia coli

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Citations

64 review citations

Antagonistic Regulation of ABA and GA in Metabolism and Signaling Pathways.
Liu et al. (2018)
63 more

4 mentions without citation

The computational-based structure of Dwarf14 provides evidence for its role as potential strigolactone receptor in plants.
Gaiji et al. (2012)
3 more