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An abnormal pK(a) value of internal histidine of the insulin molecule revealed by neutron crystallographic analysis.

Ishikawa T, Chatake T, Morimoto Y, Maeda M, Kurihara K, Tanaka I, Niimura N
Biochem Biophys Res Commun 376 32-5 (2008)
Cited: 13 times
EuropePMC logo PMID: 18725203

Abstract

Insulin is stored in pancreatic beta-cell as hexameric form with Zn2+ ions, while the hormonally active form is monomer. The hexamer requires the coordination of Zn2+ ions to the HisB10. In order to reveal the mechanism of the hexamerization of insulin, we investigated the Zn2+ free insulin at pD6.6 and pD9 by neutron crystallographic analyses. HisB10 is doubly protonated not only at pD6.6 but also at pD9, indicating an abnormal pK(a) of this histidine. It is suggested that HisB10 acts on a strong cation capture and contributes to the high stability of the hexameric form in pancreas.

Reviews - 2zpp mentioned but not cited (2)

  1. Neutron crystallography: opportunities, challenges, and limitations. Blakeley MP, Langan P, Niimura N, Podjarny A. Curr Opin Struct Biol 18 593-600 (2008)
  2. Evaluation of models determined by neutron diffraction and proposed improvements to their validation and deposition. Liebschner D, Afonine PV, Moriarty NW, Langan P, Adams PD. Acta Crystallogr D Struct Biol 74 800-813 (2018)

Articles - 2zpp mentioned but not cited (5)

  1. phenix.model_vs_data: a high-level tool for the calculation of crystallographic model and data statistics. Afonine PV, Grosse-Kunstleve RW, Chen VB, Headd JJ, Moriarty NW, Richardson JS, Richardson DC, Urzhumtsev A, Zwart PH, Adams PD. J Appl Crystallogr 43 669-676 (2010)
  2. Large-volume protein crystal growth for neutron macromolecular crystallography. Ng JD, Baird JK, Coates L, Garcia-Ruiz JM, Hodge TA, Huang S. Acta Crystallogr F Struct Biol Commun 71 358-370 (2015)
  3. X-ray structure of perdeuterated diisopropyl fluorophosphatase (DFPase): perdeuteration of proteins for neutron diffraction. Blum MM, Tomanicek SJ, John H, Hanson BL, Rüterjans H, Schoenborn BP, Langan P, Chen JC. Acta Crystallogr Sect F Struct Biol Cryst Commun 66 379-385 (2010)
  4. Neutron crystallographic refinement with REFMAC5 from the CCP4 suite. Catapano L, Long F, Yamashita K, Nicholls RA, Steiner RA, Murshudov GN. Acta Crystallogr D Struct Biol 79 1056-1070 (2023)
  5. The T2 structure of polycrystalline cubic human insulin. Triandafillidis DP, Karavassili F, Spiliopoulou M, Valmas A, Athanasiadou M, Nikolaras G, Fili S, Kontou P, Bowler MW, Chasapis CT, Von Dreele RB, Fitch AN, Margiolaki I. Acta Crystallogr D Struct Biol 79 374-386 (2023)


Reviews citing this publication (2)

  1. Neutron protein crystallography: A complementary tool for locating hydrogens in proteins. O'Dell WB, Bodenheimer AM, Meilleur F. Arch Biochem Biophys 602 48-60 (2016)
  2. The use of neutron scattering to determine the functional structure of glycoside hydrolase. Nakamura A, Ishida T, Samejima M, Igarashi K. Curr Opin Struct Biol 40 54-61 (2016)

Articles citing this publication (4)

  1. New method to compute Rcomplete enables maximum likelihood refinement for small datasets. Luebben J, Gruene T. Proc Natl Acad Sci U S A 112 8999-9003 (2015)
  2. Computational and structural evidence for neurotransmitter-mediated modulation of the oligomeric states of human insulin in storage granules. Palivec V, Viola CM, Kozak M, Ganderton TR, Křížková K, Turkenburg JP, Haluŝková P, Žáková L, Jiráĉek J, Jungwirth P, Brzozowski AM. J Biol Chem 292 8342-8355 (2017)
  3. Insulin solubility transitions by pH-dependent interactions with proinsulin C-peptide. Landreh M, Alvelius G, Willander H, Stukenborg JB, Söder O, Johansson J, Jörnvall H. FEBS J 279 4589-4597 (2012)
  4. A technique for determining the deuterium/hydrogen contrast map in neutron macromolecular crystallography. Chatake T, Fujiwara S. Acta Crystallogr D Struct Biol 72 71-82 (2016)


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