› 2zpk

X-ray diffraction
1.8Å resolution

Crystal structure of P20.1 Fab fragment in complex with its antigen peptide

Released:
DOI: 10.2210/pdb2zpk/pdb
PDB entry 2zpk coloured by chain, front view.
PDB entry 2zpk coloured by chain, side view.
PDB entry 2zpk coloured by chain, top view.
Source organisms:
Primary publication:
Novel affinity tag system using structurally defined antibody-tag interaction: application to single-step protein purification.
Nogi T, Sangawa T, Tabata S, Nagae M, Tamura-Kawakami K, Beppu A, Hattori M, Yasui N, Takagi J
Protein Sci 17 2120-6 (2008)
PMID: 18787202

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-234835 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
IgG1-lambda P20.1 Fab (light chain) Chains: L, M
Molecule details ›
Chains: L, M
Length: 212 amino acids
Theoretical weight: 22.93 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
IgG1-lambda P20.1 Fab (heavy chain) Chains: H, I
Molecule details ›
Chains: H, I
Length: 216 amino acids
Theoretical weight: 23.24 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Proteinase-activated receptor 4 Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 8 amino acids
Theoretical weight: 874 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q96RI0 (Residues: 46-53; Coverage: 2%)
Gene names: F2RL3, PAR4

Ligands and Environments

1 bound ligand:
Ligand GOL 3 x GOL
1 modified residue:
Ligand PCA 4 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P1
Unit cell:
a: 40.045Å b: 65.271Å c: 85.03Å
α: 99.93° β: 93.5° γ: 96.46°
R-values:
R R work R free
0.174 0.172 0.204
Expression system: Not provided

Quick links

Citations

2 review citations

Computer-aided antibody design.
Kuroda et al. (2012)
1 more

3 mentions without citation

Advanced glycation end product recognition by the receptor for AGEs.
Xue et al. (2011)
2 more