› 2zp1

X-ray diffraction
1.7Å resolution

Structual basis of iodo-tyrosine recognition by engineered archeal tyrosyl-tRNA synthetase

Released:
DOI: 10.2210/pdb2zp1/pdb
PDB entry 2zp1 coloured by chain, front view.
PDB entry 2zp1 coloured by chain, side view.
PDB entry 2zp1 coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Entry authors: Oki K, Kobayashi T, Sakamoto K, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176482 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 314 amino acids
Theoretical weight: 36.11 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q57834 (Residues: 1-306; Coverage: 100%)
Gene names: MJ0389, tyrS
Sequence domains: tRNA synthetases class I (W and Y)
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand IYR 1 x IYR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P43212
Unit cell:
a: 102.201Å b: 102.201Å c: 71.904Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.193 0.22
Expression system: Escherichia coli

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