2znl

X-ray diffraction
2.3Å resolution

Crystal structure of PA-PB1 complex form influenza virus RNA polymerase

Released:

Function and Biology Details

Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Polymerase acidic protein Chain: A
Molecule details ›
Chain: A
Length: 478 amino acids
Theoretical weight: 54.71 KDa
Source organism: Influenza A virus (A/Puerto Rico/8/1934(H1N1))
Expression system: Escherichia coli
UniProt:
  • Canonical: P03433 (Residues: 239-716; Coverage: 67%)
Gene name: PA
RNA-directed RNA polymerase catalytic subunit Chain: B
Molecule details ›
Chain: B
Length: 81 amino acids
Theoretical weight: 8.82 KDa
Source organism: Influenza A virus (A/Puerto Rico/8/1934(H1N1))
Expression system: Escherichia coli
UniProt:
  • Canonical: P03431 (Residues: 1-81; Coverage: 11%)
Gene name: PB1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P3221
Unit cell:
a: 101.957Å b: 101.957Å c: 115.023Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.207 0.262
Expression system: Escherichia coli