X-ray diffraction
2.2Å resolution

Crystal structure of Mycobacterium tuberculosis S-adenosyl-L-homocysteine hydrolase in ternary complex with NAD and 3-deazaadenosine


Function and Biology Details

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Adenosylhomocysteinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 495 amino acids
Theoretical weight: 54.39 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
  • Canonical: P9WGV3 (Residues: 2-495; Coverage: 100%)
Gene names: MTCY20B11.23c, Rv3248c, ahcY, sahH
Sequence domains:
Structure domains:

Ligands and Environments

Cofactor: Ligand NAD 4 x NAD
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 100.55Å b: 111.317Å c: 94.473Å
α: 90° β: 96.77° γ: 90°
R R work R free
0.202 0.198 0.285
Expression system: Escherichia coli