PDBe 2zix

X-ray diffraction
3.5Å resolution

Crystal structure of the Mus81-Eme1 complex

Released:
Source organism: Homo sapiens
Primary publication:
Crystal structure of the Mus81-Eme1 complex.
Genes Dev. 22 1093-106 (2008)
PMID: 18413719

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Crossover junction endonuclease MUS81 Chain: A
Molecule details ›
Chain: A
Length: 307 amino acids
Theoretical weight: 34.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96NY9 (Residues: 246-551; Coverage: 56%)
Gene name: MUS81
Sequence domains: ERCC4 domain
Structure domains:
Crossover junction endonuclease EME1 Chain: B
Molecule details ›
Chain: B
Length: 341 amino acids
Theoretical weight: 37.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96AY2 (Residues: 246-570; Coverage: 57%)
Gene names: EME1, MMS4
Sequence domains: ERCC4 domain
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: P3121
Unit cell:
a: 85.814Å b: 85.814Å c: 176.409Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.288 0.285 0.346
Expression system: Escherichia coli BL21(DE3)