2zhp

X-ray diffraction
1.6Å resolution

Crystal structure of bleomycin-binding protein from Streptoalloteichus hindustanus complexed with bleomycin derivative

Released:
Entry authors: Okumura H, Miyazaki I, Simizu S, Osada H

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-148074 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bleomycin resistance protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 124 amino acids
Theoretical weight: 13.81 KDa
Source organism: Streptoalloteichus hindustanus
Expression system: Escherichia coli
UniProt:
  • Canonical: P17493 (Residues: 1-124; Coverage: 100%)
Gene name: ble
Sequence domains: Glyoxalase superfamily protein
Structure domains: 2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P212121
Unit cell:
a: 39.992Å b: 53.868Å c: 125.498Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.187 0.216
Expression system: Escherichia coli