› 2zhg

X-ray diffraction
2.8Å resolution

Crystal structure of SoxR in complex with DNA

Released:
DOI: 10.2210/pdb2zhg/pdb
PDB entry 2zhg coloured by chain, front view.
PDB entry 2zhg coloured by chain, side view.
PDB entry 2zhg coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Crystal structure of the [2Fe-2S] oxidative-stress sensor SoxR bound to DNA.
Watanabe S, Kita A, Kobayashi K, Miki K
Proc Natl Acad Sci U S A 105 4121-6 (2008)
PMID: 18334645

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115966 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Redox-sensitive transcriptional activator SoxR Chain: A
Molecule details ›
Chain: A
Length: 154 amino acids
Theoretical weight: 17.18 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ACS2 (Residues: 1-154; Coverage: 100%)
Gene names: JW4024, b4063, marC, soxR
Sequence domains:
Structure domains: Multidrug-efflux Transporter Regulator; Chain: A; Domain 2
DNA (5'-D(*DGP*DCP*DCP*DTP*DCP*DAP*DAP*DGP*DTP*DTP*DAP*DAP*DCP*DTP*DTP*DGP*DAP*DGP*DGP*DC)-3') Chain: B
Molecule details ›
Chain: B
Length: 20 nucleotides
Theoretical weight: 6.13 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand FES 1 x FES
Ligand DTT 1 x DTT
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: P6122
Unit cell:
a: 53.374Å b: 53.374Å c: 355.159Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.243 0.243 0.281
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

22 review citations

Coordination chemistry of bacterial metal transport and sensing.
Ma et al. (2009)
21 more

16 mentions without citation

Transcription Factors That Defend Bacteria Against Reactive Oxygen Species.
Imlay JA. (2015)
15 more