› 2zb7

X-ray diffraction
1.8Å resolution

Crystal structure of human 15-ketoprostaglandin delta-13-reductase in complex with NADPH and nicotinamide

Released:
DOI: 10.2210/pdb2zb7/pdb
PDB entry 2zb7 coloured by chain, front view.
PDB entry 2zb7 coloured by chain, side view.
PDB entry 2zb7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for catalytic and inhibitory mechanisms of human prostaglandin reductase PTGR2.
Wu YH, Ko TP, Guo RT, Hu SM, Chuang LM, Wang AH
Structure 16 1714-23 (2008)
PMID: 19000823

Function and Biology Details

Reaction catalysed: 
11-alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)(+) = (5Z)-(13E)-11-alpha-hydroxy-9,15-dioxoprosta-5,13-dienoate + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185435 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Prostaglandin reductase 2 Chain: A
Molecule details ›
Chain: A
Length: 357 amino acids
Theoretical weight: 38.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8N8N7 (Residues: 1-351; Coverage: 100%)
Gene names: PTGR2, ZADH1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NDP 1 x NDP
1 bound ligand:
Ligand NCA 1 x NCA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 39.872Å b: 67.989Å c: 122.965Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.164 0.206
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The elusive endogenous adipogenic PPARĪ³ agonists: Lining up the suspects.
Hallenborg et al. (2016)
1 more