X-ray diffraction
2.7Å resolution

Crystal structure of the soluble domain of STT3 from P. furiosus


Function and Biology Details

Reaction catalysed:
An archaeal dolichyl phosphooligosaccharide + [protein]-L-asparagine = an archaeal dolichyl phosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
Dolichyl-phosphooligosaccharide-protein glycotransferase 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 497 amino acids
Theoretical weight: 55.98 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli BL21
  • Canonical: Q8U4D2 (Residues: 471-967; Coverage: 51%)
Gene names: PF0156, aglB1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21212
Unit cell:
a: 136.935Å b: 265.297Å c: 74.138Å
α: 90° β: 90° γ: 90°
R R work R free
0.227 0.227 0.273
Expression system: Escherichia coli BL21