2zab

X-ray diffraction
1.66Å resolution

Crystal Structure of Family 7 Alginate Lyase A1-II' Y284F in Cmplex with Product (GGG)

Released:
Source organism: Sphingomonas sp. A1
Entry authors: Ogura K, Yamasaki M, Mikami B, Hashimoto W, Murata K

Function and Biology Details

Reaction catalysed:
Eliminative cleavage of alginate to give oligosaccharides with 4-deoxy-alpha-L-erythro-hex-4-enuronosyl groups at their non-reducing ends and beta-D-mannuronate at their reducing end.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alginate_lyase2 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 25.78 KDa
Source organism: Sphingomonas sp. A1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q75WP3 (Residues: 81-308; Coverage: 74%)
Gene name: aly
Sequence domains: Alginate lyase
Structure domains: Jelly Rolls

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P212121
Unit cell:
a: 34.73Å b: 68.102Å c: 79.95Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.16 0.194
Expression system: Escherichia coli