› 2z92

X-ray diffraction
2.3Å resolution

Crystal structure of the Fab fragment of anti-ciguatoxin antibody 10C9 in complex with CTX3C_ABCDE

Released:
DOI: 10.2210/pdb2z92/pdb
PDB entry 2z92 coloured by chain, front view.
PDB entry 2z92 coloured by chain, side view.
PDB entry 2z92 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
How protein recognizes ladder-like polycyclic ethers. Interactions between ciguatoxin (CTX3C) fragments and its specific antibody 10C9.
Ui M, Tanaka Y, Tsumuraya T, Fujii I, Inoue M, Hirama M, Tsumoto K
J Biol Chem 283 19440-7 (2008)
PMID: 18463096

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-234844 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Anti-ciguatoxin antibody 10C9 Fab heavy chain Chain: A
Molecule details ›
Chain: A
Length: 218 amino acids
Theoretical weight: 23.84 KDa
Source organism: Mus musculus
Expression system: Not provided
Structure domains: Immunoglobulins
Anti-ciguatoxin antibody 10C9 Fab light chain Chain: B
Molecule details ›
Chain: B
Length: 213 amino acids
Theoretical weight: 23.22 KDa
Source organism: Mus musculus
Expression system: Not provided
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand ENE 1 x ENE
Ligand CIT 1 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P212121
Unit cell:
a: 62Å b: 67.65Å c: 114.357Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.228 0.269
Expression system: Not provided

Quick links

Citations

5 review citations

Update on methodologies available for ciguatoxin determination: perspectives to confront the onset of ciguatera fish poisoning in Europe.
Caillaud et al. (2010)
4 more

1 mention without citation

A complete, multi-level conformational clustering of antibody complementarity-determining regions.
Nikoloudis et al. (2014)