› 2z74

X-ray diffraction
2.2Å resolution

T. tengcongensis glmS ribozyme bound to glucose-6-phosphate

Released:
DOI: 10.2210/pdb2z74/pdb
PDB entry 2z74 coloured by chain, front view.
PDB entry 2z74 coloured by chain, side view.
PDB entry 2z74 coloured by chain, top view.
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis
Primary publication:
Requirement of helix P2.2 and nucleotide G1 for positioning the cleavage site and cofactor of the glmS ribozyme.
Klein DJ, Wilkinson SR, Been MD, Ferré-D'Amaré AR
J Mol Biol 373 178-89 (2007)
PMID: 17804015

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-113109 (preferred)
Entry contents:
1 distinct RNA molecule
1 distinct DNA/RNA hybrid molecule
Macromolecules (2 distinct):
glmS ribozyme RNA Chain: B
Molecule details ›
Chain: B
Length: 125 nucleotides
Theoretical weight: 40.54 KDa
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis
Expression system: Not provided
Sequence domains: glmS glucosamine-6-phosphate activated ribozyme
glmS ribozyme substrate RNA Chain: A
Molecule details ›
Chain: A
Length: 27 nucleotides
Theoretical weight: 8.59 KDa
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand G6P 1 x G6P
Ligand MG 8 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21212
Unit cell:
a: 181.11Å b: 40.06Å c: 70.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.209 0.235
Expression system: Not provided

Quick links

Citations

15 review citations

The structural and functional diversity of metabolite-binding riboswitches.
Roth et al. (2009)
14 more

6 mentions without citation

Riboswitches as Drug Targets for Antibiotics.
Panchal et al. (2021)
5 more