PDBe 2z56

X-ray diffraction
1.9Å resolution

Crystal structure of G56S-propeptide:S324A-subtilisin complex

Released:

Function and Biology Details

Reaction catalysed:
Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides.
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tk-subtilisin Chain: A
Molecule details ›
Chain: A
Length: 318 amino acids
Theoretical weight: 32.87 KDa
Source organism: Thermococcus kodakarensis KOD1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P58502 (Residues: 105-422; Coverage: 80%)
Gene name: TK1675
Sequence domains: Subtilase family
Structure domains: Peptidase S8/S53 domain
Tk-subtilisin Chain: B
Molecule details ›
Chain: B
Length: 65 amino acids
Theoretical weight: 7.11 KDa
Source organism: Thermococcus kodakarensis KOD1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P58502 (Residues: 29-93; Coverage: 16%)
Gene name: TK1675
Structure domains: Peptidase S8 propeptide/proteinase inhibitor I9

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P212121
Unit cell:
a: 64.518Å b: 67.463Å c: 74.046Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.189 0.21
Expression system: Escherichia coli BL21(DE3)