PDBe 2z3n

X-ray diffraction
2.5Å resolution

complex structure of LF-transferase and peptide B

Released:

Function and Biology Details

Reaction catalysed:
L-leucyl-tRNA(Leu) + N-terminal L-arginyl-[protein] = tRNA(Leu) + N-terminal L-leucyl-L-arginyl-[protein]
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Leucyl/phenylalanyl-tRNA--protein transferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 233 amino acids
Theoretical weight: 26.52 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A8P1 (Residues: 2-234; Coverage: 100%)
Gene names: JW0868, aat, b0885, ycaA
Sequence domains: Leucyl/phenylalanyl-tRNA protein transferase
Structure domains:
peptide (PHE)(ARG)(TYR)(LEU)(GLY) Chains: C, D
Molecule details ›
Chains: C, D
Length: 5 amino acids
Theoretical weight: 656 Da
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P21212
Unit cell:
a: 114.936Å b: 130.101Å c: 38.353Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.222 0.259
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided