› 2z1y

X-ray diffraction
1.75Å resolution

Crystal structure of LysN, alpha-aminoadipate aminotransferase (complexed with N-(5'-phosphopyridoxyl)-L-leucine), from Thermus thermophilus HB27

Released:
DOI: 10.2210/pdb2z1y/pdb
PDB entry 2z1y coloured by chain, front view.
PDB entry 2z1y coloured by chain, side view.
PDB entry 2z1y coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Entry authors: Tomita T, Miyazaki T, Miyagawa T, Fushinobu S, Kuzuyama T, Nishiyama M

Function and Biology Details

Reaction catalysed: 
L-2-aminoadipate + 2-oxoglutarate = 2-oxoadipate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180893 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-aminoadipate transaminase Chains: A, B
Molecule details ›
Chains: A, B
Length: 397 amino acids
Theoretical weight: 43.9 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: Q72LL6 (Residues: 1-397; Coverage: 100%)
Gene names: TT_C0043, lysN
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
1 bound ligand:
Ligand LEU 2 x LEU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: C2
Unit cell:
a: 137.107Å b: 62.229Å c: 107.439Å
α: 90° β: 116.34° γ: 90°
R-values:
R R work R free
0.18 0.18 0.217
Expression system: Escherichia coli

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