› 2z0j

X-ray diffraction
1.5Å resolution

Crystal structure of uncharacterized conserved protein from Thermus thermophilus HB8

Released:
DOI: 10.2210/pdb2z0j/pdb
PDB entry 2z0j coloured by chain, front view.
PDB entry 2z0j coloured by chain, side view.
PDB entry 2z0j coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Nakagawa N, Kukimoto-Niino M, Yokoyama S, Kuramitsu S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
(2R)-2-phospho-3-sulfolactate + H(2)O = (2R)-3-sulfolactate + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177952 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable 2-phosphosulfolactate phosphatase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 237 amino acids
Theoretical weight: 24.77 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SID6 (Residues: 1-237; Coverage: 100%)
Gene names: TTHA1438, comB
Sequence domains: 2-phosphosulpholactate phosphatase
Structure domains: ComB-like

Ligands and Environments

2 bound ligands:
Ligand CA 9 x CA
Ligand MES 4 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL45XU
Spacegroup: P21
Unit cell:
a: 89.86Å b: 65.004Å c: 176.266Å
α: 90° β: 97.83° γ: 90°
R-values:
R R work R free
0.188 0.188 0.211
Expression system: Escherichia coli BL21(DE3)

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