X-ray diffraction
1.9Å resolution

The crystal structure of human Atg4B- LC3(1-124) complex


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cysteine protease ATG4B Chain: A
Molecule details ›
Chain: A
Length: 357 amino acids
Theoretical weight: 40.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
  • Canonical: Q9Y4P1 (Residues: 1-353; Coverage: 90%)
Gene names: APG4B, ATG4B, AUTL1, KIAA0943
Sequence domains: Peptidase family C54
Microtubule-associated proteins 1A/1B light chain 3B Chain: B
Molecule details ›
Chain: B
Length: 129 amino acids
Theoretical weight: 14.93 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21
  • Canonical: Q62625 (Residues: 1-124; Coverage: 87%)
Gene names: Map1alc3, Map1lc3, Map1lc3b
Sequence domains: Autophagy protein Atg8 ubiquitin like
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 46.85Å b: 90.883Å c: 102.472Å
α: 90° β: 90° γ: 90°
R R work R free
0.203 0.2 0.229
Expression system: Escherichia coli BL21