2ywf

X-ray diffraction
2.24Å resolution

Crystal structure of GMPPNP-bound LepA from Aquifex aeolicus

Released:
Source organism: Aquifex aeolicus VF5
Entry authors: Kawazoe M, Takemoto C, Kaminishi T, Nishino A, Nakayama-Ushikoshi R, Hanawa-Suetsugu K, Terada T, Shirouzu M, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Elongation factor 4 Chain: A

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: C2221
Unit cell:
a: 81.451Å b: 116.142Å c: 123.329Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.251
Expression system: Escherichia coli BL21(DE3)