X-ray diffraction
2.7Å resolution

Crystal Structure of Aspartate Semialdehyde Dehydrogenase from Thermus thermophilus HB8

Source organism: Thermus thermophilus HB8
Entry authors: Kagawa W, Fujikawa N, Kurumizaka H, Bessho Y, Ellis MJ, Antonyuk SV, Strange RW, Hasnain SS, Kuramitsu S, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed:
L-aspartate 4-semialdehyde + phosphate + NADP(+) = L-4-aspartyl phosphate + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Aspartate-semialdehyde dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 331 amino acids
Theoretical weight: 36.12 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
  • Canonical: Q5SKU8 (Residues: 1-331; Coverage: 100%)
Gene names: TTHA0545, asd
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P3121
Unit cell:
a: 125.167Å b: 125.167Å c: 116.463Å
α: 90° β: 90° γ: 120°
R R work R free
0.368 0.303 0.368
Expression system: Escherichia coli