2yr4

X-ray diffraction
1.7Å resolution

Crystal structure of L-phenylalanine oxiase from Psuedomonas sp. P-501

Released:

Function and Biology Details

Reaction catalysed:
L-phenylalanine + O(2) = 2-phenylacetamide + CO(2) + H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phenylalanine 2-monooxygenase precursor Chains: A, B
Molecule details ›
Chains: A, B
Length: 721 amino acids
Theoretical weight: 77.9 KDa
Source organism: Pseudomonas sp. P-501
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5W9R9 (Residues: 2-714; Coverage: 100%)
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P21212
Unit cell:
a: 141.577Å b: 145.405Å c: 82.076Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.166 0.195
Expression system: Escherichia coli BL21(DE3)