Structure analysis

Structure of the SCL:E47 complex bound to DNA

X-ray diffraction
2.87Å resolution
Source organism: Homo sapiens
Assembly composition:
hetero tetramer (preferred)
Entry contents: 2 distinct polypeptide molecules
2 distinct DNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero tetramer
Accessible surface area: 12900 Å2
Buried surface area: 5500 Å2
Dissociation area: 1,200 Å2
Dissociation energy (ΔGdiss): 18 kcal/mol
Dissociation entropy (TΔSdiss): 11 kcal/mol
Interface energy (ΔGint): -54 kcal/mol
Symmetry number: 1

Macromolecules

Chain: A
Length: 91 amino acids
Theoretical weight: 10.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P17542 (Residues: 180-253; Coverage: 22%)
Gene names: BHLHA17, SCL, TAL1, TCL5
Pfam: Helix-loop-helix DNA-binding domain
InterPro:
CATH: Helix-loop-helix DNA-binding domain

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Chain: B
Length: 82 amino acids
Theoretical weight: 9.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P15923 (Residues: 538-616; Coverage: 12%)
  • Best match: P15923-2 (Residues: 535-613)
Gene names: BHLHB21, E2A, ITF1, TCF3
Pfam: Helix-loop-helix DNA-binding domain
InterPro:
CATH: Helix-loop-helix DNA-binding domain

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Name: EBOX FORWARD
Representative chains: E
Length: 11 nucleotides
Theoretical weight: 3.28 KDa

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Name: EBOX REVERSE
Representative chains: F
Length: 11 nucleotides
Theoretical weight: 3.42 KDa

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