› 2yjp

X-ray diffraction
2.26Å resolution

Crystal structure of the solute receptors for L-cysteine of Neisseria gonorrhoeae

Released:
DOI: 10.2210/pdb2yjp/pdb
PDB 2yjp coloured by chain and viewed from the front.
PDB 2yjp coloured by chain and viewed from the side.
PDB 2yjp coloured by chain and viewed from the top.
Source organism: Neisseria gonorrhoeae FA 1090
Primary publication:
Crystal structures of two solute receptors for L-cystine and L-cysteine, respectively, of the human pathogen Neisseria gonorrhoeae.
Bulut H, Moniot S, Licht A, Scheffel F, Gathmann S, Saenger W, Schneider E
J Mol Biol 415 560-72 (2012)
PMID: 22138345

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PBPb domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 291 amino acids
Theoretical weight: 31.4 KDa
Source organism: Neisseria gonorrhoeae FA 1090
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5F5B5 (Residues: 18-284; Coverage: 100%)
Gene name: NGO_2014
Sequence domains: Bacterial extracellular solute-binding proteins, family 3
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

3 bound ligands:
Ligand CYS 3 x CYS
Ligand ZN 8 x ZN
Ligand EDO 6 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 58.71Å b: 91.59Å c: 158.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.185 0.233
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Cysteine biosynthesis in Neisseria species.
Hicks et al. (2018)
1 more