Solution NMR

Model of human U2AF65 tandem RRM1 and RRM2 domains with eight-site uridine binding


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Splicing factor U2AF 65 kDa subunit Chain: A
Molecule details ›
Chain: A
Length: 198 amino acids
Theoretical weight: 21.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
  • Canonical: P26368 (Residues: 148-342; Coverage: 41%)
Gene names: U2AF2, U2AF65
Sequence domains: RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Structure domains: Alpha-Beta Plaits
5'-R(*UP*UP*UP*UP*UP*UP*UP*UP*UP)-3' Chain: B
Molecule details ›
Chain: B
Length: 9 nucleotides
Theoretical weight: 2.71 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 55%
Refinement method: MODIFIED ARIA
Chemical shifts: BMR17623  
Expression systems:
  • Escherichia coli BL21
  • Not provided