› 2yh1

Solution NMR

Model of human U2AF65 tandem RRM1 and RRM2 domains with eight-site uridine binding

Released:
DOI: 10.2210/pdb2yh1/pdb
PDB 2yh1 coloured by chain and viewed from the front.
PDB 2yh1 coloured by chain and viewed from the side.
PDB 2yh1 coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Multi-domain conformational selection underlies pre-mRNA splicing regulation by U2AF.
Mackereth CD, Madl T, Bonnal S, Simon B, Zanier K, Gasch A, Rybin V, Valcárcel J, Sattler M
Nature 475 408-11 (2011)
PMID: 21753750

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Splicing factor U2AF 65 kDa subunit Chain: A
Molecule details ›
Chain: A
Length: 198 amino acids
Theoretical weight: 21.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P26368 (Residues: 148-342; Coverage: 41%)
Gene names: U2AF2, U2AF65
Sequence domains: RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Structure domains: Alpha-Beta Plaits
5'-R(*UP*UP*UP*UP*UP*UP*UP*UP*UP)-3' Chain: B
Molecule details ›
Chain: B
Length: 9 nucleotides
Theoretical weight: 2.71 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 55%
Refinement method: MODIFIED ARIA
Chemical shifts: BMR17623  
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

20 review citations

Integrated structural biology to unravel molecular mechanisms of protein-RNA recognition.
Schlundt et al. (2017)
19 more

7 mentions without citation

Investigating the Role of Large-Scale Domain Dynamics in Protein-Protein Interactions.
Delaforge et al. (2016)
6 more