› 2yey

X-ray diffraction
4.5Å resolution

Crystal structure of the allosteric-defective chaperonin GroEL E434K mutant

Released:
DOI: 10.2210/pdb2yey/pdb
PDB entry 2yey coloured by chain, front view.
PDB entry 2yey coloured by chain, side view.
PDB entry 2yey coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Crystal structure of the temperature-sensitive and allosteric-defective chaperonin GroELE461K.
Cabo-Bilbao A, Spinelli S, Sot B, Agirre J, Mechaly AE, Muga A, Guérin DM
J Struct Biol 155 482-92 (2006)
PMID: 16904907

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + a folded polypeptide = ADP + phosphate + an unfolded polypeptide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetradecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141315 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chaperonin GroEL Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Length: 524 amino acids
Theoretical weight: 55.22 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A6F5 (Residues: 2-525; Coverage: 96%)
Gene names: JW4103, b4143, groEL, groL, mopA
Sequence domains: TCP-1/cpn60 chaperonin family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P32
Unit cell:
a: 171.908Å b: 171.908Å c: 454.592Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.165 0.167 0.24
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Structure and allostery of the chaperonin GroEL.
Saibil et al. (2013)
2 more

1 mention without citation

Cognitive impairments in a Down syndrome model with abnormal hippocampal and prefrontal dynamics and cytoarchitecture.
Muza et al. (2023)