2y7g

X-ray diffraction
1.4Å resolution

Crystal structure of the 3-keto-5-aminohexanoate cleavage enzyme (Kce) from C. Cloacamonas acidaminovorans in complex with the product acetoacetate

Released:

Function and Biology Details

Reaction catalysed:
(5S)-5-amino-3-oxohexanoate + acetyl-CoA = L-3-aminobutanoyl-CoA + acetoacetate
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-keto-5-aminohexanoate cleavage enzyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 282 amino acids
Theoretical weight: 31.09 KDa
Source organism: Candidatus Cloacimonas acidaminovorans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B0VHH0 (Residues: 2-276; Coverage: 100%)
Gene names: CLOAM0912, kce
Sequence domains: beta-keto acid cleavage enzyme
Structure domains: Aldolase class I

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P43212
Unit cell:
a: 102.1Å b: 102.1Å c: 100.736Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.167 0.186
Expression system: Escherichia coli BL21(DE3)